2Y3Q
1.55A structure of apo bacterioferritin from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 208.000, 208.000, 142.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.230 - 1.550 |
| R-factor | 0.16652 |
| Rwork | 0.165 |
| R-free | 0.20007 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vxi |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.649 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.200 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.100 | 0.620 |
| Number of reflections | 438427 | |
| <I/σ(I)> | 12.8 | 3 |
| Completeness [%] | 98.4 | 99.2 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 1.8M AMMONIUM SULPHATE, 100MM ACETATE, PH 5.5 |
