2Y2B
crystal structure of AmpD in complex with reaction products
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 32 |
| Unit cell lengths | 67.774, 67.774, 92.836 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.1734 |
| Rwork | 0.170 |
| R-free | 0.22350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y28 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.121 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.180 |
| Number of reflections | 31507 | |
| <I/σ(I)> | 13.8 | 4.8 |
| Completeness [%] | 95.2 | 77 |
| Redundancy | 4.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1M TRIS PH 6.0, 0.1M LI2SO4, 28% PEG 3350 |
