2Y1Z
Human alphaB Crystallin ACD R120G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.730, 60.730, 100.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.270 - 2.500 |
| R-factor | 0.18047 |
| Rwork | 0.173 |
| R-free | 0.24052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HUMAN ALPHA-CRYSTALLIN DOMAIN RESIDUES 71- 157 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.964 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.540 |
| Number of reflections | 7582 | |
| <I/σ(I)> | 19.4 | 4.3 |
| Completeness [%] | 98.0 | 97.8 |
| Redundancy | 9.2 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | PROTEIN AT 16.5 MG/ML IN BUFFER (25 MM TRISHCL, PH 7.5, 25 MM NACL) WAS MIXED 1:1 WITH MOTHER LIQUOR (36% MPD, 0.18M POTASSIUM CHLORIDE) |
