2Y1T
Bacillus subtilis prophage dUTPase in complex with dUDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-10 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.515, 99.338, 99.306 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.310 - 1.890 |
| R-factor | 0.142 |
| Rwork | 0.140 |
| R-free | 0.16600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHAIN A OF PDB ENTRY 2XX6 |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.323 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0086) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.450 |
| Number of reflections | 78511 | |
| <I/σ(I)> | 25.4 | 3.1 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES BUFFER PH 7.5, 10% (V/V) PEG 8000, 8% ETHYLENE GLYCOL |
