2Y1L

Caspase-8 in Complex with DARPin-8.4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2010-04-23
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	61.000, 81.600, 163.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.857 - 1.800
R-factor	0.182
Rwork	0.180
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1QDU AND 2QYJ
RMSD bond length	0.009
RMSD bond angle	1.201
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.860	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.100	0.510
Number of reflections	75862
<I/σ(I)>	13.29	3.76
Completeness [%]	99.4	98.9
Redundancy	4.9	4.88

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.8		100 MM CITRIC ACID PH 4.9 (RT), 200 MM LI2SO4, 22.5% PEG 4K.