2Y0N
CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN DOSAGE COMPENSATION FACTORS MSL1 AND MSL3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-26 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.230, 127.270, 79.680 |
| Unit cell angles | 90.00, 118.46, 90.00 |
Refinement procedure
| Resolution | 46.130 - 3.000 |
| R-factor | 0.23337 |
| Rwork | 0.232 |
| R-free | 0.25295 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f5j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.118 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0085) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 3.120 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.030 | 0.520 |
| Number of reflections | 25057 | |
| <I/σ(I)> | 16.37 | 2.04 |
| Completeness [%] | 93.5 | 94.9 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M ADA (PH 6.5), 0.1M LI2SO4, 0.9M MGSO4. |
