2Y02
TURKEY BETA1 ADRENERGIC RECEPTOR WITH STABILISING MUTATIONS AND BOUND AGONIST CARMOTEROL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.600, 61.700, 101.400 |
| Unit cell angles | 90.00, 109.30, 90.00 |
Refinement procedure
| Resolution | 95.730 - 2.600 |
| R-factor | 0.23142 |
| Rwork | 0.229 |
| R-free | 0.26828 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vt4 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.391 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.300 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.130 | 0.600 |
| Number of reflections | 31463 | |
| <I/σ(I)> | 7.5 | 1.9 |
| Completeness [%] | 97.1 | 93.6 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | SITTING DROP VAPOUR DIFFUSION, PROTEIN (16MG/ML) IN 100MM NACL, 0.1MM EDTA, 1.0MM (R, R)-CARMOTEROL, 1.9MG/ML CHS, 0.65% HEGA-10, 0.1M BICINE PH9.0, 26% PEG600 AT 4 DEGREES C |
