2Y01
TURKEY BETA1 ADRENERGIC RECEPTOR WITH STABILISING MUTATIONS AND BOUND PARTIAL AGONIST DOBUTAMINE (CRYSTAL DOB102)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-26 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.100, 61.600, 101.400 |
Unit cell angles | 90.00, 108.90, 90.00 |
Refinement procedure
Resolution | 95.920 - 2.600 |
R-factor | 0.22772 |
Rwork | 0.226 |
R-free | 0.26636 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vt4 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.385 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.900 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.130 | 0.720 |
Number of reflections | 30953 | |
<I/σ(I)> | 7.1 | 1.9 |
Completeness [%] | 95.6 | 91.3 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | SITTING DROP VAPOUR DIFFUSION, PROTEIN (15MG/ML) IN 100MM NACL, 0.1MM EDTA, 1.0MM (R, S)-DOBUTAMINE, 0.9MG/ML CHS, 0.6% HEGA-10, 0.1M BICINE PH9, 25% PEG600 AT 4 DEGREES C, pH 9.0 |