2XZ0
The Structure of the 2:1 (Partially Occupied) Complex Between Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean) and Acyl Carrier Protein.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MAR555 FLAT PANEL |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 188.274, 188.274, 81.315 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.280 - 3.000 |
| R-factor | 0.24434 |
| Rwork | 0.243 |
| R-free | 0.27744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.299 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.080 | 0.480 |
| Number of reflections | 33376 | |
| <I/σ(I)> | 21.9 | 4.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.1 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.62 | 4-5% PEG 20000, 8% PEG 550MME, 180MM SODIUM BROMIDE, 100MM SODIUM ACETATE PH 5.62, 15% GLYCEROL. |
