2XYU
Crystal structure of EphA4 kinase domain in complex with VUF 12058
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.544, 91.545, 97.959 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.187 - 2.117 |
| R-factor | 0.1916 |
| Rwork | 0.189 |
| R-free | 0.22980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y6m |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.024 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.190 | 2.230 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.140 | 0.690 |
| Number of reflections | 17378 | |
| <I/σ(I)> | 8.5 | 1.9 |
| Completeness [%] | 99.5 | 98.2 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | AMMONIUM SULFATE, BISTRIS PH 5.5, PEG 10K |
