2XXV
Crystal structure of the GluK2 (GluR6) M770K LBD dimer in complex with kainate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 96.260, 104.873, 113.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.939 - 1.700 |
| R-factor | 0.1669 |
| Rwork | 0.165 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xxt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.085 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.810 |
| Number of reflections | 55807 | |
| <I/σ(I)> | 15.6 | 2.1 |
| Completeness [%] | 88.1 | 91.5 |
| Redundancy | 7.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP 27% PEG 4000, 6% PROPAN-2-OL, 80MM SODIUM ACETATE |
