2XWP
ANAEROBIC COBALT CHELATASE (CbiK) FROM SALMONELLA TYPHIMURIUM IN COMPLEX WITH METALATED TETRAPYRROLE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 110.630, 110.630, 89.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.770 - 1.900 |
| R-factor | 0.1965 |
| Rwork | 0.194 |
| R-free | 0.23928 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qgo |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.248 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | 0.700 |
| Number of reflections | 176900 | |
| <I/σ(I)> | 10.4 | 2.6 |
| Completeness [%] | 99.4 | 97.4 |
| Redundancy | 8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
