2XVZ
Cobalt chelatase CbiK (periplasmatic) from Desulvobrio vulgaris Hildenborough (co-crystallized with cobalt)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-12 |
Detector | MARRESEARCH |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 121.441, 121.441, 121.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 85.750 - 2.400 |
R-factor | 0.17211 |
Rwork | 0.170 |
R-free | 0.22022 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.020 |
RMSD bond angle | 1.989 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.450 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.067 | 0.411 |
Number of reflections | 18046 | |
<I/σ(I)> | 10.9 | 1.9 |
Completeness [%] | 97.5 | 96.6 |
Redundancy | 7.7 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | CRYSTALLIZATION SOLUTION: 100MM TRIS-HCL PH8.5, 2M AMMONIUM SULFATE. THE DROP WAS MADE BY ADDING 1UL OF PROTEIN PLUS 1.8UL OF CRYSTALLIZATION SOLUTION AND 0.2UL OF 1M COBALT CHLORIDE. |