2XVS
Crystal structure of human TTC5 (Strap) C-terminal OB domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.260, 62.390, 78.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.770 - 1.800 |
| R-factor | 0.1755 |
| Rwork | 0.172 |
| R-free | 0.23638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.524 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.300 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 | 0.500 |
| Number of reflections | 14246 | |
| <I/σ(I)> | 8.7 | 2.1 |
| Completeness [%] | 98.6 | 91 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.18M SODIUM IODIDE, 0.02M SODIUM ACETATE, 20% PEG3350, 10% ETHYLENE GLYCOL, 0.45% DMSO, 0.1M BIS-TRIS-PROPANE PH8.5 |
