2XV4
Structure of Human RPC62 (partial)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.540, 69.920, 75.970 |
| Unit cell angles | 90.00, 112.84, 90.00 |
Refinement procedure
| Resolution | 70.010 - 2.950 |
| R-factor | 0.2294 |
| Rwork | 0.228 |
| R-free | 0.25610 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 3.070 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.040 | 0.040 |
| Number of reflections | 16477 | |
| <I/σ(I)> | 20.61 | 3.53 |
| Completeness [%] | 91.9 | 78.7 |
| Redundancy | 3.71 | 3.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | BTP PH 7.5 GLYCEROL 10% PEG 3350 TACSIMATE 12% |
