2XUM
FACTOR INHIBITING HIF (FIH) Q239H MUTANT IN COMPLEX WITH ZN(II), NOG AND ASP-SUBSTRATE PEPTIDE (20-MER)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-25 |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.352, 86.352, 144.533 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.120 - 2.200 |
R-factor | 0.20228 |
Rwork | 0.201 |
R-free | 0.22178 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h2k |
RMSD bond length | 0.007 |
RMSD bond angle | 1.034 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS (1.1) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.120 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | 0.880 |
Number of reflections | 26863 | |
<I/σ(I)> | 28.5 | 2.23 |
Completeness [%] | 99.1 | 98 |
Redundancy | 9.2 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 1.6M AMMONIUM SULPHATE, 6% PEG400, 0.1M HEPES PH7.5, VAPOR DIFFUSION, SITTING DROP |