2XSS
Crystal structure of GAFb from the human phosphodiesterase 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.750, 64.750, 145.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.390 - 2.500 |
| R-factor | 0.25187 |
| Rwork | 0.248 |
| R-free | 0.31709 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.552 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.390 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.640 |
| Number of reflections | 12657 | |
| <I/σ(I)> | 27 | 5.2 |
| Completeness [%] | 99.0 | 98.5 |
| Redundancy | 11.9 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 16% PEG 3350, 0.06 M CITRIC ACID, 0.04 M BIS-TRIS PROPANE |
