2XSG
Structure of the gh92 family glycosyl hydrolase ccman5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-18 |
Detector | MARRESEARCH |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 82.202, 91.158, 224.523 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.800 - 2.000 |
R-factor | 0.19303 |
Rwork | 0.191 |
R-free | 0.23861 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.237 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.800 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.160 | 0.400 |
Number of reflections | 106820 | |
<I/σ(I)> | 7.8 | 3.7 |
Completeness [%] | 97.6 | 89.6 |
Redundancy | 5.7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.2 M NAF, 0.1 M BIS-TRIS PROPANE PH 7.5, 20% PEG 3350 |