2XR9

Crystal structure of Autotaxin (ENPP2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	53.808, 63.289, 70.471
Unit cell angles	98.78, 106.22, 99.77

Refinement procedure

Resolution	19.980 - 2.050
R-factor	0.175
Rwork	0.173
R-free	0.22053
Structure solution method	SAD
Starting model (for MR)	2gsn
RMSD bond length	0.016
RMSD bond angle	1.628
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.100	0.380
Number of reflections	52153
<I/σ(I)>	13.9	2.49
Completeness [%]	96.8	93.9
Redundancy	4.8	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1