2XR5
Crystal structure of the complex of the carbohydrate recognition domain of human DC-SIGN with pseudo dimannoside mimic.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-23 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.450, 71.450, 52.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.410 - 1.420 |
| R-factor | 0.14954 |
| Rwork | 0.148 |
| R-free | 0.17113 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2it6 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.553 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.410 | 1.460 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.130 | 0.580 |
| Number of reflections | 25925 | |
| <I/σ(I)> | 28.67 | 5.56 |
| Completeness [%] | 99.4 | 92.4 |
| Redundancy | 25.4 | 7.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | VAPOR DIFFUSION, HANGING DROP, 293K. 20% PEG 3350, 200 MM NACL, 100 MM CACODYLATE PH 6.5, CRYOPROTECTED IN PARATONE-N. |
