2XQH
Crystal structure of an immunoglobulin-binding fragment of the trimeric autotransporter adhesin EibD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 48.950, 48.950, 409.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.512 - 1.990 |
| R-factor | 0.1599 |
| Rwork | 0.159 |
| R-free | 0.18550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p9h |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.027 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.510 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.030 | 0.090 |
| Number of reflections | 24632 | |
| <I/σ(I)> | 30.61 | 5.89 |
| Completeness [%] | 97.8 | 84.2 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.0 M AMMONIUM PHOSPHATE, 0.1 M SODIUM CITRATE PH 5.6 |
