1P9H

CRYSTAL STRUCTURE OF THE COLLAGEN-BINDING DOMAIN OF YERSINIA ADHESIN YadA

Summary for 1P9H

• Entry DOI: 10.2210/pdb1p9h/pdb
• Descriptor: Invasin (2 entities in total)
• Functional Keywords: collagen-binding, left-handed beta-roll, cell adhesion
• Biological source: Yersinia enterocolitica
• Cellular location: Cell outer membrane: P31489
• Total number of polymer chains: 1
• Total formula weight: 22806.06

Authors

Nummelin, H.,Merckel, M.C.,Skurnik, M.,Goldman, A. (deposition date: 2003-05-12, release date: 2004-03-23, Last modification date: 2024-02-14)

Primary citation

Nummelin, H.,Merckel, M.C.,Leo, J.C.,Lankinen, H.,Skurnik, M.,Goldman, A.
The Yersinia adhesin YadA collagen-binding domain structure is a novel left-handed parallel beta-roll.
Embo J., 23:701-711, 2004

PubMed Abstract: The crystal structure of the recombinant collagen-binding domain of Yersinia adhesin YadA from Yersinia enterocolitica serotype O:3 was solved at 1.55 A resolution. The trimeric structure is composed of head and neck regions, and the collagen binding head region is a novel nine-coiled left-handed parallel beta-roll. Before the beta-roll, the polypeptide loops from one monomer to the rest, and after the beta-roll the neck region does the same, making the transition from the globular head region to the narrower stalk domain. This creates an intrinsically stable 'lock nut' structure. The trimeric form of YadA is required for collagen binding, and mutagenesis of its surface residues allowed identification of a putative collagen-binding surface. Furthermore, a new structure-sequence motif for YadA beta-roll was used to identify putative YadA-head-like domains in a variety of human and plant pathogens. Such domains may therefore be a common bacterial strategy for avoiding host response.

PubMed: 14765110
DOI: 10.1038/sj.emboj.7600100

Experimental method

X-RAY DIFFRACTION (1.55 Å)