2XQ4
Pentameric ligand gated ion channel GLIC in complex with tetramethylarsonium (TMAs)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2009-10-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.314, 128.310, 164.374 |
| Unit cell angles | 90.00, 104.04, 90.00 |
Refinement procedure
| Resolution | 40.200 - 3.600 |
| R-factor | 0.2599 |
| Rwork | 0.259 |
| R-free | 0.27220 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.500 |
| High resolution limit [Å] | 3.400 |
| Rmerge | 0.080 |
| Number of reflections | 50589 |
| <I/σ(I)> | 12.4 |
| Completeness [%] | 89.1 |
| Redundancy | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 9% PEG 4000, 50 MM CH3COONA, 200 MM (NH4)2SO4, PH 4.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K. |
