2XQ1
Crystal structure of peroxisomal catalase from the yeast Hansenula polymorpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-11 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 132.520, 196.680, 170.850 |
Unit cell angles | 90.00, 92.85, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
R-factor | 0.19076 |
Rwork | 0.191 |
R-free | 0.22297 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a4e |
RMSD bond length | 0.014 |
RMSD bond angle | 1.471 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.430 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.120 | 0.570 |
Number of reflections | 193014 | |
<I/σ(I)> | 10.9 | 2.5 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2 M MAGNESIUM FORMATE, 20% (W/V) PEG 3350 |