2XQ1
Crystal structure of peroxisomal catalase from the yeast Hansenula polymorpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 132.520, 196.680, 170.850 |
| Unit cell angles | 90.00, 92.85, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.19076 |
| Rwork | 0.191 |
| R-free | 0.22297 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a4e |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.471 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.430 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.120 | 0.570 |
| Number of reflections | 193014 | |
| <I/σ(I)> | 10.9 | 2.5 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MAGNESIUM FORMATE, 20% (W/V) PEG 3350 |
