2XPX
Crystal structure of BHRF1:Bak BH3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 62.387, 62.387, 93.729 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.950 - 2.050 |
| R-factor | 0.18505 |
| Rwork | 0.184 |
| R-free | 0.21696 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v6q |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.843 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.060 | 0.500 |
| Number of reflections | 12945 | |
| <I/σ(I)> | 33.9 | 2.2 |
| Completeness [%] | 99.5 | 95.8 |
| Redundancy | 10 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.4 | 1.6 M NANO3, 50 MM MALIC ACID PH 4.4 |
