2XNM
Structure of NEK2 bound to CCT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-23 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.800, 56.830, 81.300 |
| Unit cell angles | 90.00, 133.21, 90.00 |
Refinement procedure
| Resolution | 36.734 - 1.850 |
| R-factor | 0.1649 |
| Rwork | 0.163 |
| R-free | 0.19620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.022 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.170 | 1.890 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 27237 | |
| <I/σ(I)> | 11.1 | 2.5 |
| Completeness [%] | 98.1 | 99.1 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
