2XN9
Crystal structure of the ternary complex between human T cell receptor, staphylococcal enterotoxin H and human major histocompatibility complex class II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-23 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.180, 48.890, 166.720 |
| Unit cell angles | 90.00, 113.55, 90.00 |
Refinement procedure
| Resolution | 152.500 - 2.300 |
| R-factor | 0.22414 |
| Rwork | 0.222 |
| R-free | 0.27236 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1OGA CHAINS D E AND PDB ENTRY 1HXY CHAINS A B D |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.443 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.720 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.450 |
| Number of reflections | 61858 | |
| <I/σ(I)> | 10.3 | 2.1 |
| Completeness [%] | 97.6 | 88.8 |
| Redundancy | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 15% W/V PEG 5000 MME, 0.1 M BIS-TRIS PH 7.0, 0.1 M NACL |
