2XN3
Crystal structure of thyroxine-binding globulin complexed with mefenamic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Collection date | 2007-10-11 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 172.835, 41.624, 56.188 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.390 - 2.090 |
| R-factor | 0.21284 |
| Rwork | 0.210 |
| R-free | 0.25957 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2riv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.258 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.970 | 2.210 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.090 | 0.320 |
| Number of reflections | 24692 | |
| <I/σ(I)> | 14.5 | 3.3 |
| Completeness [%] | 98.2 | 88.5 |
| Redundancy | 5.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.4 | 20% PEG3350, PH 5.4 |
