2XKG
C.lacteus mini-Hb Leu86Ala mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.650, 43.347, 59.443 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.250 - 1.600 |
| R-factor | 0.15529 |
| Rwork | 0.154 |
| R-free | 0.18581 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kr7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.172 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.900 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.100 |
| Number of reflections | 15045 | |
| <I/σ(I)> | 24.1 | 12.7 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 277 | 60 % AMMONIUM SULFATE AND 50 MM SODIUM ACETATE BUFFER, PH 6, AT 4C |
