2XK5
Crystal structure of K6-linked diubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 104.840, 104.840, 104.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.947 - 3.000 |
| R-factor | 0.2179 |
| Rwork | 0.213 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jf5 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.771 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.410 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.070 | 0.580 |
| Number of reflections | 4247 | |
| <I/σ(I)> | 13.5 | 2.6 |
| Completeness [%] | 99.0 | 98.6 |
| Redundancy | 4.9 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 19-20 % PEG 3350, 0.2 M ZINC ACETATE, pH 7 |
