2XIK
Structure of Human YSK1 (Yeast Sps1-Ste20-related Kinase 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.080, 48.560, 67.570 |
| Unit cell angles | 90.00, 113.78, 90.00 |
Refinement procedure
| Resolution | 22.340 - 1.970 |
| R-factor | 0.1907 |
| Rwork | 0.188 |
| R-free | 0.23310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.7.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.230 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.050 | 0.620 |
| Number of reflections | 23929 | |
| <I/σ(I)> | 7.8 | 2 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M K3(CIT),0.1M BTPROP 8.5, 20% PEG3350,10% ETGLY |
