2XEF
Human glutamate carboxypeptidase II in complex with Antibody- Recruiting Molecule ARM-P8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-21 |
| Detector | MARRESEARCH MX-300 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 101.844, 130.026, 158.741 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.590 |
| R-factor | 0.16107 |
| Rwork | 0.161 |
| R-free | 0.18266 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oot |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.692 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 137748 | |
| <I/σ(I)> | 15.4 | 2.6 |
| Completeness [%] | 96.9 | 77.9 |
| Redundancy | 6.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 33% (V/V) PENTAERYTHRITOL PROPOXYLATE PO/OH 5/4, 1% (W/V) PEG 3350, 100 MM TRIS-HCL, pH 8.0 |
