2XDV
Crystal Structure of the Catalytic Domain of FLJ14393
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2007-08-21 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 185.180, 185.180, 185.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.280 - 2.570 |
R-factor | 0.1981 |
Rwork | 0.197 |
R-free | 0.22850 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | BUSTER-TNT (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.700 |
High resolution limit [Å] | 2.560 | 2.560 |
Rmerge | 0.090 | 1.000 |
Number of reflections | 35135 | |
<I/σ(I)> | 17 | 2 |
Completeness [%] | 99.5 | 97 |
Redundancy | 9.4 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 12% PEG 3350; 0.005M COCL2; 0.005M MGCL2; 0.005M CDCL2; 0.005M NICL2; 0.1M HEPES PH 7.5 |