2XDP
Crystal structure of the tudor domain of human JMJD2C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-20 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 52.815, 79.646, 81.614 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.512 - 1.560 |
| R-factor | 0.1868 |
| Rwork | 0.186 |
| R-free | 0.20640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gfa |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.240 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.580 | 1.640 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.060 | 0.510 |
| Number of reflections | 24650 | |
| <I/σ(I)> | 15 | 1.9 |
| Completeness [%] | 99.1 | 94.9 |
| Redundancy | 5.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M (NH4)2SO4; 0.1M TRIS PH 8.5; 25% PEG 3350 |
