2XD4
Nucleotide-bound Structures of Bacillus subtilis Glycinamide Ribonucleotide Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-28 |
| Detector | RIGAKU RAXIS-IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.990, 84.410, 85.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.220 - 2.650 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xcl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.210 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.100 | 0.220 |
| Number of reflections | 11710 | |
| <I/σ(I)> | 22.6 | |
| Completeness [%] | 93.4 | 66.4 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% PEG 4000, 0.1 M TRIS-HCL PH 8.5, 4.2 MM MGCL2, 1.8 MM ATP |
