2XCV
Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II in complex with inosine monophosphate and 2,3-bisphosphoglycerate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2008-01-30 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 91.570, 127.790, 130.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.680 - 2.300 |
R-factor | 0.17893 |
Rwork | 0.176 |
R-free | 0.22868 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jcm |
RMSD bond length | 0.021 |
RMSD bond angle | 1.849 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.070 | 0.410 |
Number of reflections | 34216 | |
<I/σ(I)> | 17.8 | 4.6 |
Completeness [%] | 99.4 | 99.5 |
Redundancy | 5.4 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |