2XBF
Nedd4 HECT structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-22 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.405, 38.843, 60.640 |
| Unit cell angles | 90.00, 93.13, 90.00 |
Refinement procedure
| Resolution | 48.576 - 2.503 |
| R-factor | 0.1997 |
| Rwork | 0.197 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oni |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.603 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.410 |
| Number of reflections | 14288 | |
| <I/σ(I)> | 16.2 | 2.3 |
| Completeness [%] | 99.4 | 94.1 |
| Redundancy | 3.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 100 MM NA-MES, PH 6.0, 4% PEG 400, 35 MM CACL2, 5 MM TCEP |
