2XA0

Crystal structure of BCL-2 in complex with a BAX BH3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	93
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 31 2 1
Unit cell lengths	81.973, 81.973, 138.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.2223
Rwork	0.222
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g5m
RMSD bond length	0.009
RMSD bond angle	1.363
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.080	0.220
Number of reflections	14540
<I/σ(I)>	30.4	4.5
Completeness [%]	93.9	75.3
Redundancy	9.7	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M SODIUM ACETATE (PH 4.2), 3.0 M SODIUM FORMATE