2X9Q
Structure of the Mycobacterium tuberculosis protein, Rv2275, demonstrates that cyclodipeptide synthetases are related to type I tRNA-Synthetases.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 193 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.090, 75.060, 140.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.180 - 2.020 |
| R-factor | 0.18142 |
| Rwork | 0.180 |
| R-free | 0.21047 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.047 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.410 |
| Number of reflections | 49827 | |
| <I/σ(I)> | 13.5 | 3.6 |
| Completeness [%] | 98.9 | 95.9 |
| Redundancy | 5.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.75 | 12-15% (W/V) PEG 8000, 1M LICL, 100 MM TRIS/BICINE, PH 8.75 |
