2X8F

Native structure of Endo-1,5-alpha-L-arabinanases from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	110
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	51.975, 57.645, 86.272
Unit cell angles	82.31, 87.97, 63.66

Refinement procedure

Resolution	19.620 - 1.900
R-factor	0.143
Rwork	0.141
R-free	0.18000
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.015
RMSD bond angle	1.471
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.500	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.050	0.140
Number of reflections	67116
<I/σ(I)>	14.5
Completeness [%]	95.7	87.1
Redundancy	2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1