2X7V

Crystal structure of Thermotoga maritima endonuclease IV in the presence of zinc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-16
Detector	MARRESEARCH MX-300
Spacegroup name	P 61
Unit cell lengths	123.370, 123.370, 35.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.614 - 2.300
R-factor	0.1488
Rwork	0.146
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qtw
RMSD bond length	0.009
RMSD bond angle	1.103
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.610	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.060	0.180
Number of reflections	13865
<I/σ(I)>	4.5	2.1
Completeness [%]	99.4	99.9
Redundancy	4.2	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9		0.1 M SODIUM CHLORIDE, 0.1 M BICINE PH 9.0, 20%(V/V) POLYETHYLENE GLYCOL MONOMETHYL ETHER 550, 10 MM ZINC SULFATE