2X7O
Crystal structure of TGFbRI complexed with an indolinone inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2004-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 179.929, 246.561, 131.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.720 - 3.700 |
| R-factor | 0.2673 |
| Rwork | 0.267 |
| R-free | 0.27320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ias |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.890 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.700 | 3.900 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.020 | 0.070 |
| Number of reflections | 24744 | |
| <I/σ(I)> | 4.7 | 1.9 |
| Completeness [%] | 79.2 | 81 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 1.5 M AMMONIUM SULFATE, 0.1 M GLYCINE PH 7.25 |
