2X7H
Crystal structure of the human MGC45594 gene product in complex with fenoprofen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.600, 52.050, 75.730 |
| Unit cell angles | 94.28, 91.29, 102.18 |
Refinement procedure
| Resolution | 75.460 - 1.600 |
| R-factor | 0.16831 |
| Rwork | 0.168 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wek |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.458 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.520 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.090 | 0.460 |
| Number of reflections | 84512 | |
| <I/σ(I)> | 10.4 | 1.9 |
| Completeness [%] | 92.4 | 90.5 |
| Redundancy | 4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.5M NA MALONATE PH7.0, 0.25W/V JEFFAMINE ED 2001_PH7.0, 8PH HEPES. |
