2X7G
Structure of human serine-arginine-rich protein-specific kinase 2 (SRPK2) bound to purvalanol B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-28 |
| Detector | RAYONIX |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 110.310, 110.310, 90.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.000 - 2.500 |
| R-factor | 0.18615 |
| Rwork | 0.183 |
| R-free | 0.24868 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wbp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.368 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.210 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.130 | 0.590 |
| Number of reflections | 22306 | |
| <I/σ(I)> | 6.5 | 2 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.75 | 0.5M AMMONIUM SULPHATE, 0.1M SODIUM ACETATE PH4.75 |
