2X7E
Crystal structure of human kinesin Eg5 in complex with (R)-fluorastrol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-02-12 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.597, 79.849, 160.063 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.19983 |
| Rwork | 0.196 |
| R-free | 0.27323 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x88 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.819 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.330 |
| Number of reflections | 35497 | |
| Completeness [%] | 95.5 | 93.4 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 30% PEG3350, 0.2M K2HPO4, 0.1M HEPES PH8.0 |
