2X7A
Structural basis of HIV-1 tethering to membranes by the Bst2-tetherin ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-01 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.890, 85.930, 123.310 |
| Unit cell angles | 90.00, 126.94, 90.00 |
Refinement procedure
| Resolution | 44.880 - 2.770 |
| R-factor | 0.2383 |
| Rwork | 0.236 |
| R-free | 0.27280 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.241 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.920 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.090 | 0.480 |
| Number of reflections | 35553 | |
| <I/σ(I)> | 13.8 | 3.8 |
| Completeness [%] | 98.1 | 97.7 |
| Redundancy | 7.6 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.02M MGCL2, 0.1M BIS TRIS PH5.0, 20% POLYACRYLIC ACID |
