2X6N
Human foamy virus integrase - catalytic core. Manganese-bound structure.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2009-01-28 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.767, 89.232, 177.084 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.620 - 2.060 |
R-factor | 0.22793 |
Rwork | 0.226 |
R-free | 0.27004 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x74 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.085 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.230 | 2.170 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.070 | 0.700 |
Number of reflections | 78830 | |
<I/σ(I)> | 14.2 | 2 |
Completeness [%] | 93.8 | 71 |
Redundancy | 3.5 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1.8-2M AMMONIUM FORMATE 100MM HEPES, PH 7.5 5MM MNCL2 10-15% GLYCEROL |