2X6J
THE CRYSTAL STRUCTURE OF THE DROSOPHILA CLASS III PI3-KINASE VPS34 IN COMPLEX WITH PIK-93
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-11 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 109.950, 156.330, 242.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.620 - 3.500 |
R-factor | 0.232 |
Rwork | 0.230 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x6h |
RMSD bond length | 0.011 |
RMSD bond angle | 1.658 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.280 | 3.690 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.110 | 0.450 |
Number of reflections | 26700 | |
<I/σ(I)> | 4.8 | 1.18 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 3.52 | 3.64 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | PROTEIN WAS CRYSTALLISED IN 0.88M AMMONIUM SULPHATE, 100MM DI-POTASSIUM HYDROGEN PHOSPHATE AND 100MM DI-SODIUM HYDROGEN PHOSPHATE (TITRATED TO PH 7.5 WITH ORTHOPHOSPHORIC ACID). PROTEIN WAS SOAKED WITH 0.5MM PIK-93 IN MOTHER LIQUOR. |