2X6J
THE CRYSTAL STRUCTURE OF THE DROSOPHILA CLASS III PI3-KINASE VPS34 IN COMPLEX WITH PIK-93
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-11 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 109.950, 156.330, 242.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.620 - 3.500 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x6h |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.658 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.280 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.110 | 0.450 |
| Number of reflections | 26700 | |
| <I/σ(I)> | 4.8 | 1.18 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.52 | 3.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN WAS CRYSTALLISED IN 0.88M AMMONIUM SULPHATE, 100MM DI-POTASSIUM HYDROGEN PHOSPHATE AND 100MM DI-SODIUM HYDROGEN PHOSPHATE (TITRATED TO PH 7.5 WITH ORTHOPHOSPHORIC ACID). PROTEIN WAS SOAKED WITH 0.5MM PIK-93 IN MOTHER LIQUOR. |
