2X6F
THE CRYSTAL STRUCTURE OF THE DROSOPHILA CLASS III PI3-KINASE VPS34 IN COMPLEX WITH 3-METHYLADENINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-11 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 112.070, 155.140, 244.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.130 - 3.300 |
R-factor | 0.245 |
Rwork | 0.242 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x6h |
RMSD bond length | 0.011 |
RMSD bond angle | 1.647 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.920 | 3.480 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.100 | 0.690 |
Number of reflections | 31665 | |
<I/σ(I)> | 4.99 | 1.04 |
Completeness [%] | 98.2 | 99.7 |
Redundancy | 3.6 | 3.66 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | PROTEIN WAS CRYSTALLISED IN 0.88M AMMONIUM SULPHATE, 100MM DI-POTASSIUM HYDROGEN PHOSPHATE AND 100MM DI-SODIUM HYDROGEN PHOSPHATE (TITRATED TO PH 7.5 WITH ORTHOPHOSPHORIC ACID). PROTEIN WAS SOAKED WITH 1MM 3-MA IN MOTHER LIQUOR. |