2X4T
Crystal structure of MHC CLass I HLA-A2.1 bound to a Peiodate- cleavable peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2007-12-08 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.391, 85.516, 80.394 |
Unit cell angles | 90.00, 90.33, 90.00 |
Refinement procedure
Resolution | 19.830 - 2.300 |
R-factor | 0.1858 |
Rwork | 0.184 |
R-free | 0.23570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eey |
RMSD bond length | 0.008 |
RMSD bond angle | 1.334 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.500 | 2.420 |
High resolution limit [Å] | 2.200 | 2.300 |
Rmerge | 0.060 | 0.510 |
Number of reflections | 37003 | |
<I/σ(I)> | 7.9 | 1.4 |
Completeness [%] | 98.1 | 97.9 |
Redundancy | 3.1 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.1 M MES PH 6.5, 22% PEG1500 |